Resource Type

Journal Article 91

Conference Videos 2

Year

2023 3

2022 11

2021 6

2020 4

2019 4

2018 6

2017 2

2016 3

2015 4

2014 4

2013 6

2012 4

2011 3

2010 8

2009 7

2008 3

2007 7

2006 1

2004 2

2003 2

open ︾

Keywords

polymorphism 8

Polymorphism 2

biomarker 2

bond-energy 2

bond-length 2

force constant 2

terminal restriction fragment length polymorphism 2

1 860 MPa 1

10kV high voltage electric cable 1

2′-O-methylation 1

4 250 m 1

Bemisia tabaci 1

Gossypium genotypes 1

Poncirus trifoliata 1

k-? model 1

HhaI 1

MspI 1

ALS/FTD 1

ANSYS-secondary-development 1

open ︾

Search scope:

排序： Display mode:

Current molecular biologic techniques for characterizing environmental microbial community

Dawen GAO, Yu TAO

Frontiers of Environmental Science & Engineering 2012, Volume 6, Issue 1,   Pages 82-97 doi: 10.1007/s11783-011-0306-6

Abstract: Microbes are vital to the earth because of their enormous numbers and instinct function maintaining the natural balance. Since the microbiology was applied in environmental science and engineering more than a century ago, researchers desire for more and more information concerning the microbial spatio-temporal variations in almost every fields from contaminated soil to wastewater treatment plant (WWTP). For the past 30 years, molecular biologic techniques explored for environmental microbial community (EMC) have spanned a broad range of approaches to facilitate the researches with the assistance of computer science: faster, more accurate and more sensitive. In this feature article, we outlined several current and emerging molecular biologic techniques applied in detection of EMC, and presented and assessed in detail the application of three promising tools.

Keywords： molecular biological technique     microbial community     denaturing gradient gel electrophoresis (DGGE)     terminalrestriction fragment length polymorphism (T-RFLP)     environmental applications    

HTML PDF Collect

Spatial and seasonal variations in bacterial communities of the Yellow Sea by T-RFLP analysis

Hongyuan WANG, Xiaolu JIANG, Ya HE, Huashi GUAN

Frontiers of Environmental Science & Engineering 2009, Volume 3, Issue 2,   Pages 194-199 doi: 10.1007/s11783-009-0018-3

Abstract: This was accomplished by using terminal restriction fragment length polymorphism (T-RFLP) analysis ofTwo kinds of tetrameric restriction enzymes, I and I, were used in the experiment to depict the bacterial

Keywords： terminal restriction fragment length polymorphism (T-RFLP)     bacterial community structure     marine microbial    

HTML PDF Collect

Diversity and vertical distributions of sediment bacteria in an urban river contaminated by nutrients and heavy metals

Xunan YANG, Shan HUANG, Qunhe WU, Renduo ZHANG, Guangli LIU

Frontiers of Environmental Science & Engineering 2013, Volume 7, Issue 6,   Pages 851-859 doi: 10.1007/s11783-013-0569-1

Abstract: The bacterial OTUs were measured using the terminal restriction fragment length polymorphism analysis

Keywords： heavy metals     nutrients     sediment profile     terminal restriction fragment length polymorphism    

HTML PDF Collect

Response of bacterial communities to short-term pyrene exposure in red soil

Jingjing PENG, Hong LI, Jianqiang SU, Qiufang ZHANG, Junpeng RUI, Chao CAI

Frontiers of Environmental Science & Engineering 2013, Volume 7, Issue 4,   Pages 559-567 doi: 10.1007/s11783-013-0501-8

Abstract: The bacterial communities in the incubated soils were analyzed using 16S rRNA sequencing and terminalrestriction fragment length polymorphism (T-RFLP) methods.

Keywords： pyrene     bacterial communities     terminal restriction fragment length polymorphism     short-term exposure     rank-abundance    

HTML PDF Collect

Community dynamics of ammonia oxidizing bacteria in a full-scale wastewater treatment system with nitrification stability

Xiaohui WANG, Xianghua WEN, Hengjing YAN, Kun DING, Man HU

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 1,   Pages 92-98 doi: 10.1007/s11783-010-0254-6

Abstract: The community dynamics were investigated using specific PCR followed by terminal restriction fragmentlength polymorphism (T-RFLP) analysis of the gene.

Keywords： ammonia-oxidizing bacteria (AOB)     community dynamics     terminal restriction fragment length polymorphism    

HTML PDF Collect

Association study on GNB3 gene polymorphism with essential hypertension in Xinjiang Uygur group

JING Jianying, WANG Dan, WANG Xiaofeng, JIN Jianzhong, JIN Li, JIAO Yi, WEN Hao, LIN Renyong

Frontiers of Medicine 2007, Volume 1, Issue 2,   Pages 230-233 doi: 10.1007/s11684-007-0045-z

Abstract: The relationship between the tenth exon C825T of G-protein β3 subunit (GNB) genetic polymorphism andPolymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to genotypeGNB C825T polymorphism in 354 hypertensive (HT) and 384 normotensive (NT) Uygur subjects.No evidence was found to suggest an association between GNB C825T polymorphism and hypertension in the

Keywords： case-control     significant difference     reaction-restriction fragment     C825T polymorphism     evidence    

HTML PDF Collect

Calorie restriction and its impact on gut microbial composition and global metabolism

Xiaojiao Zheng, Shouli Wang, Wei Jia

Frontiers of Medicine 2018, Volume 12, Issue 6,   Pages 634-644 doi: 10.1007/s11684-018-0670-8

Abstract:

Calorie restriction (CR) is a dietary regimen that reduces calorie intake without incurring malnutrition

Keywords： caloric restriction     gut microbiota     metabolome    

HTML PDF Collect

CD176 single-chain variable antibody fragment inhibits the adhesion of cancer cells to endothelial cells

Jiangnan Liu,Bin Yi,Zhe Zhang,Yi Cao

Frontiers of Medicine 2016, Volume 10, Issue 2,   Pages 204-211 doi: 10.1007/s11684-016-0443-1

Abstract: A positive clone of CD176 single-chain variable antibody fragment (scFv) was successfully screened from

Keywords： CD176     Thomsen-Friedenreich antigen     scFv     cancer     therapy     adhesion     metastasis    

HTML PDF Collect

Proteomics study of Mycoplasma pneumoniae pneumonia reveals the Fc fragment of the IgG-binding protein

Frontiers of Medicine 2022, Volume 16, Issue 3,   Pages 378-388 doi: 10.1007/s11684-021-0840-y

Abstract: The Fc fragment of the IgG-binding protein (FCGBP) was identified as the most promising indicator of

Keywords： severe Mycoplasma pneumoniae pneumonia     children     proteomics     Fc fragment of the IgG-binding protein    

HTML PDF Collect

Association of SIPA1 545 C>T polymorphism with survival in Chinese women with metastatic breast cancer

Renling Pei, Ye Xu, Yan Wei, Tao Ouyang, Jinfeng Li, Tianfeng Wang, Zhaoqing Fan, Tie Fan, Benyao Lin, Yuntao Xie

Frontiers of Medicine 2013, Volume 7, Issue 1,   Pages 138-142 doi: 10.1007/s11684-013-0247-5

Abstract: The purpose of this study was to determine whether SIPA1 545 C>T polymorphism was associatedIn this study, SIPA1 545 C>T polymorphism was detected in 185 metastatic breast cancer patientsusing polymerase chain reaction-restriction fragment length polymorphism assay (PCR-RFLP).Survival curves for patients with SIPA1 545 C>T polymorphism was compared using the Kaplan-MeierWe found that SIPA1 545 C>T polymorphism was significantly associated with survival in 185

Keywords： SIPA1     polymorphism     metastatic breast cancer     survival    

HTML PDF Collect

The role of natriuretic peptide precursor A gene polymorphism in the development of coronary heart disease

Ripen NSENGA MD, Longxian CHENG PhD, Mei’an HE PhD, Tangchun WU PhD,

Frontiers of Medicine 2009, Volume 3, Issue 4,   Pages 437-442 doi: 10.1007/s11684-009-0074-x

Abstract: Rs5065 NPPA gene polymorphism leads to the translation of NPPA with two additional arginines and hasThe purpose of the present study was to investigate the relationship between the rs5065 NPPA gene polymorphismWe genotyped the single nucleotide polymorphism (SNP) rs5065 NPPA in the human NPPA gene in 1861 sex-Our results suggest that the C allele of rs5065 NPPA gene polymorphism may be associated with the risk

Keywords： natriuretic peptide precursor A     coronary heart disease     gene polymorphism     allelic discrimination     polymorphism    

HTML PDF Collect

Polymorphism of pharmaceutical molecules: perspectives on nucleation

Jie LU, Zhen LI, Xiaolin JIANG,

Frontiers of Chemical Science and Engineering 2010, Volume 4, Issue 1,   Pages 37-44 doi: 10.1007/s11705-009-0294-2

Abstract: Polymorphism is a widespread phenomenon observed in more than half of all drug substances.

Keywords： development     Polymorphism     elucidation     controversial     relationship    

HTML PDF Collect

Effects of slip length and hydraulic diameter on hydraulic entrance length of microchannels with superhydrophobic

Wenchi GONG, Jun SHEN, Wei DAI, Zeng DENG, Xueqiang DONG, Maoqiong GONG

Frontiers in Energy 2020, Volume 14, Issue 1,   Pages 127-138 doi: 10.1007/s11708-020-0661-8

Abstract: This paper investigated effects of slip length and hydraulic diameter on the hydraulic entrance lengthIt is found that superhydrophobic microchannels have a longer hydraulic entrance length than that ofThe dimensionless hydraulic entrance length slightly increases with the increasing slip length at approximatelyA new correlation to predict the entrance length in square microchannels with different slip lengths

Keywords： laminar flow     hydraulic entrance length     super hydrophobic surface     slip length     hydraulic diameter    

HTML PDF Collect

Genetic association between the polymorphism of cytosolic PLA2 gene family and schizophrenia

Qiong YU PhD, Xiang-Fei MENG PhD, Jie-Ping SHI, Ya-Qin YU PhD,

Frontiers of Medicine 2010, Volume 4, Issue 1,   Pages 101-105 doi: 10.1007/s11684-010-0017-6

Abstract: This study aims to investigate the genetic association between the polymorphism of cytosolic phospholipase

Keywords： schizophrenia     cytosolic phospholipase A2     ligase detection reaction     polymorphism    

HTML PDF Collect

Title Author Date Type Operation

Current molecular biologic techniques for characterizing environmental microbial community

Dawen GAO, Yu TAO

Journal Article

Spatial and seasonal variations in bacterial communities of the Yellow Sea by T-RFLP analysis

Hongyuan WANG, Xiaolu JIANG, Ya HE, Huashi GUAN

Journal Article

Diversity and vertical distributions of sediment bacteria in an urban river contaminated by nutrients and heavy metals

Xunan YANG, Shan HUANG, Qunhe WU, Renduo ZHANG, Guangli LIU

Journal Article

Response of bacterial communities to short-term pyrene exposure in red soil

Jingjing PENG, Hong LI, Jianqiang SU, Qiufang ZHANG, Junpeng RUI, Chao CAI

Journal Article

Community dynamics of ammonia oxidizing bacteria in a full-scale wastewater treatment system with nitrification stability

Xiaohui WANG, Xianghua WEN, Hengjing YAN, Kun DING, Man HU

Journal Article

Association study on GNB3 gene polymorphism with essential hypertension in Xinjiang Uygur group

JING Jianying, WANG Dan, WANG Xiaofeng, JIN Jianzhong, JIN Li, JIAO Yi, WEN Hao, LIN Renyong

Journal Article

Calorie restriction and its impact on gut microbial composition and global metabolism

Xiaojiao Zheng, Shouli Wang, Wei Jia

Journal Article

CD176 single-chain variable antibody fragment inhibits the adhesion of cancer cells to endothelial cells

Jiangnan Liu,Bin Yi,Zhe Zhang,Yi Cao

Journal Article

Key technological innovations in the construction of the main terminal of Beijing Daxing International

Journal Article

Proteomics study of Mycoplasma pneumoniae pneumonia reveals the Fc fragment of the IgG-binding protein

Journal Article

Association of SIPA1 545 C>T polymorphism with survival in Chinese women with metastatic breast cancer

Renling Pei, Ye Xu, Yan Wei, Tao Ouyang, Jinfeng Li, Tianfeng Wang, Zhaoqing Fan, Tie Fan, Benyao Lin, Yuntao Xie

Journal Article

The role of natriuretic peptide precursor A gene polymorphism in the development of coronary heart disease

Ripen NSENGA MD, Longxian CHENG PhD, Mei’an HE PhD, Tangchun WU PhD,

Journal Article

Polymorphism of pharmaceutical molecules: perspectives on nucleation

Jie LU, Zhen LI, Xiaolin JIANG,

Journal Article

Effects of slip length and hydraulic diameter on hydraulic entrance length of microchannels with superhydrophobic

Wenchi GONG, Jun SHEN, Wei DAI, Zeng DENG, Xueqiang DONG, Maoqiong GONG

Journal Article

Genetic association between the polymorphism of cytosolic PLA2 gene family and schizophrenia

Qiong YU PhD, Xiang-Fei MENG PhD, Jie-Ping SHI, Ya-Qin YU PhD,

Journal Article